《植物生理学报》 2017, 53(2): 159-166

TRV介导的VIGS技术在果蔬基因功能研究中的应用

闵德栋, 张新华^*, 季娜娜, 李富军, 邵淑君

山东理工大学农业工程与食品科学学院, 山东淄博255000

通信作者：张新华；E-mail: zxh@sdut.edu.cn

摘　要：

病毒诱导的基因沉默(virus-induced gene silencing, VIGS)是近年来开发的一种快速鉴定植物基因功能的反向遗传学技术。因该技术需要借助病毒载体来实现, 所以病毒载体的开发一直备受关注。在已开发的病毒载体中, 烟草脆裂病毒(Tobacco rattle virus, TRV)具有寄主范围广、沉默效率高、可在多个组织产生沉默, 并且病毒诱导的症状轻微等独特的优势。因此, TRV-VIGS技术已成功用于多种果蔬作物基因功能的研究。本文就TRV载体的结构特点、作用机制、在果蔬基因功能研究中的应用及存在的问题等进行了分析和总结。

关键词：病毒诱导的基因沉默; 烟草脆裂病毒; 果蔬; 基因功能

收稿：2016-09-18   修定：2016-12-12

资助：国家自然科学基金(31201432和31101587)。

The application of TRV-mediated VIGS technique in the study of gene function in fruits and vegetables

MIN De-Dong, ZHANG Xin-Hua^*, JI Na-Na, LI Fu-Jun, SHAO Shu-Jun

School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, Shandong 255000, China

Corresponding author: ZHANG Xin-Hua; E-mail: zxh@sdut.edu.cn

Abstract:

Virus-induced gene silencing (VIGS) is a recently developed reverse genetics tool for gene function analysis in diverse plant species. The technology is limited by the host range of the virus upon which the viral vector is based; therefore, the development of viral vector has always been concerned. Compared with other viruses that have been developed as VIGS vectors, TRV vectors are used in a broad host range with high silencing efficiency and can silence genes in most plant parts. Moreover, the overall symptoms of infection by TRV are very mild compared with other viruses. Therefore, the TRV-based VIGS technology has been successfully used to study the genes function in various fruits and vegetables. In this manuscript, we describe the features of TRV vector, mechanisms of TRV-VIGS and its application to gene function analysis in fruits and vegetables. Meanwhile, the problems and its solutions of the application of TRV-VIGS in fruits and vegetables were analyzed.

Key words: virus-induced gene silencing; Tobacco rattle virus; fruit and vegetable; gene function